Spectrophotometric Method Validation for the Quantification of Ethyl Alcohol in Alcoholic Beverages

Authors

  • Jayaprakash Bhoi 8th Sem, B. Pharm Student, BKCP Nuapada, Odisha, INDIA.
  • Maheswar Sa 8th Sem, B. Pharm Student, BKCP Nuapada, Odisha, INDIA.
  • Umakanta Dandasena 8th Sem, B. Pharm Student, BKCP Nuapada, Odisha, INDIA.
  • Tusharkanta Sahu 8th Sem, B. Pharm Student, BKCP Nuapada, Odisha, INDIA.
  • Akshya Kumar Mishra Associate Professor, Department of Microbiology, BKCP Nuapada, Odisha, INDIA.
  • Shibani Raut Assistant Professor, Department of Pharmaceutical Analysis and Quality Assurance, BKCP Nuapada, Odisha, INDIA.

DOI:

https://doi.org/10.55544/jrasb.4.3.15

Keywords:

Ethanol quantification, spectrophotometry, alcoholic beverages, calibration curve, forensic analysis, quality control, sensitivity, precision, LOD, LOQ

Abstract

This study presents the development and validation of a spectrophotometric method for ethanol quantification in alcoholic beverages. The method exhibited high accuracy, precision, and sensitivity, making it a reliable alternative to conventional chromatographic techniques. A strong linear correlation was observed between ethanol concentration and absorbance (R² = 0.9987), with a calibration equation of A = 0.620C + 0.005. The method was applied to whisky, vodka, and rum (each labeled as 42.5% v/v ethanol), and the measured values closely matched the expected concentrations, confirming its accuracy. Precision tests demonstrated minimal relative standard deviations (%RSD < 2%), ensuring method reliability. Sensitivity analysis revealed a Limit of Detection (LOD) of 0.018% v/v and a Limit of Quantification (LOQ) of 0.054% v/v, proving the method's capability to detect trace ethanol levels. The method is particularly useful for forensic and regulatory applications, quality control in the beverage industry, and resource-limited laboratories due to its cost-effectiveness and minimal reagent usage. Future studies may explore its application in diverse alcoholic beverages, bioethanol analysis, and portable spectrophotometric devices for on-site testing.

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Published

2025-06-26

How to Cite

Bhoi, J., Sa, M., Dandasena, U., Sahu, T., Mishra, A. K., & Raut, S. (2025). Spectrophotometric Method Validation for the Quantification of Ethyl Alcohol in Alcoholic Beverages. Journal for Research in Applied Sciences and Biotechnology, 4(3), 143–149. https://doi.org/10.55544/jrasb.4.3.15

Issue

Vol. 4 No. 3 (2025): June Issue

Section

Articles

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Copyright (c) 2025 Jayaprakash Bhoi, Maheswar Sa, Umakanta Dandasena, Tusharkanta Sahu, Akshya Kumar Mishra, Shibani Raut

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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