Effectiveness of Brucellosis on Polymorphisms of IL-1B and IL-10 Concentrations Among Iraqi Patients

Bushra Qasim Dhumad

doi:10.55544/jrasb.2.6.34

Authors

Bushra Qasim Dhumad Department of Medical, College of health and medical techniques, Baghdad, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.2.6.34

Keywords:

Brucellosis, Polymorphisms, IL-1B, IL-10

Abstract

In this study, (60) blood samples were taken from patients infected with brucellosis, and (60) samples were taken from healthy individual as a control group. The patients were visiting Emam Ali Hospital for Fever in Baghdad city, during the period from 1^st February to 1^st November 2023. The results of gender distribution showed that among patients infected with Brucellosis, 30 (50.0%) were males and 30 (50%) were females, while among the control group, 31(51.7%) were males and 29(48.3%) were females. Also the distribution of infections according residency were matched between urban and rural locations 30(50.0%) for both. While the distribution of Brucellosis among age groups showed that the highest incidence of infection was in the age groups (40-50) years and (17-29) years 22(36.7%) for each, followed by the age group (30-39) years 16(26.7%). The mean levels of anti Brucella antibodies IgM, IgG, IL-10, IL-1B in patients with brucellosis were (0.11±0.17), (0.09±0.15), (5.62±1.99), (4.92±1.76) respectively compared to their mean levels in the control group (2.01±1.17), (1.33±0.59), (60.68±26.81), (22.85±9.17) respectively with highly significant differences (p<0.01). The ROC test showed that the Sensitivity of Brucella IgM concentration was100% and Specificity 100% in Cut off >0.84. Also the Sensitivity of Brucella IgG concentration was 100% and Specificity 100% in Cut off >0.33. Also the Sensitivity of IL-10 concentration was 100% and Specificity 100% in Cut off >18.04, and the Sensitivity of IL-1B concentration was 100% and Specificity 100% in Cut off >9.08. There were mutations occurred with IL-1B gene ID 3553 in SNPs, rs1143627. The variation of wild TT was changed to CC, CC, TC, TC, CC, AG, CC, TC in 8 cases out of 11 in comparison with the control group, and the mutation occurred with IL-1B gene ID 3553 in SNPs, rs1143627. The variation of wild TT was changed to CC, CC, TC, TC, CC, AG, CC, TC in 8 cases out of 11 in comparison with the control group.

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