Typing and Integron Distribution among Multi-Drug Resistance Escherichia coli

Authors

  • Husam Ahmed AL-Nuaeyme College of Education for Pure Science, Diyala University, IRAQ.
  • Munim R. Ali College of Science, Mustansiriyah University, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.3.1.35

Keywords:

taxonomic, Escherichia coli, PCR-Integron system

Abstract

The study aimed, through the use of taxonomic associations, to find the genetic relationship between strains of Escherichia coli bacteria in 152 samples of infection symptoms. The results of the susceptibility test for 42 isolates showed that 8 isolates were sensitive to most antibiotics, while 12 isolates had moderate resistance, ranging from 5-8 antagonists, while 22 isolates were resistant to 9-12 antibiotics. Through the use of phylo-group profiling system it was found that the vast majority of Escherichia coli isolates under study have high virulence factors and possess large genomes by belonging to group B2. The results of genotyping using the PCR-Integron system showed that the unty Pable isolates were only... and that the highest percentage was in the first type, which reached... which indicates that there is a relationship between resistance to multiple antibiotics and the presence of introns.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Abass S K, Munim R and Ali H Sawsan (2014) Authman Isolation of Multi Antibiotics Resistance Escherichia coli from urinary tract infection and the Detection of PapCand fimHvirulence genes by Polymerase chain reaction Technique. Diyala jour. for pure science 10(1).

Adwan K, Jarrar N, Abu-Hijleh A, Adwan G and Awwad E (2014) Molecular characterization of Escherichia coli isolates from patients with urinary tract infections in Palestine. Journal of Medical Microbiology 63, 229–234.

Ahangarkani,F.;Rajabnia,R.;Shahandashti,E.F.;Bagheri,M.andRamez,M.(2014). Frequency of Class 1 Integron in Escherichia coli Strains Isolated from Patients withUrinary Tract Infections in North of Iran. Msm. 27:10-12.

Barlow, R.S. (2004). Isolation and Characterization of Integron-Containing Bacteria without Antibiotic Selection. Antimicrob Agents Chemother. 48: 838-842.

Bashir,S.; Haque,A.; Sarwar,Y. and Raza,A.(2015). Prevalence of Integrons and AntbiotcResistance among Uropathogenic Escherichia coli from Faisalabad Region of Pakistan. Archives of Clinical Microbiology.6 (4):9.

Betteridge T, Partridge S R, Iredell J R and Stokes H W (2011) Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive care unit. Antimicrob Agents Chemother 55, 3939–3943.

Bexiga, R .; Koskinen, M.T.; Holopainen, J.; Carneiro, C.; Pereira, H.; Ellis, K.A. and Vilela, C.L. ( 2011) . Diagnosis of intramammary infection in samples yielding negative results or minor pathogens in conventional bacterial culturing. J. Dairy Res., 78:

Cambray, G.; Guerout, AM. and Mazel D. (2010).Integrons. Annu Rev Genet. 44:141–166.

Chess T (2012) Microb. 18th edition. McGrow Hill.United State.

CLSI (Clinical & Laboratory Standards institute) (2016) Performance standard for antimicrobial susceptibility testing; Seventeenth informational supple -ment. M100-S17.Vol. 27 Pp:1.

El-Sokkary,M.M.A. and Abdelmegeed,E.S.(2015). Characterisation of Class 1 Integronamong Escherichia coli Isolated from Mansoura University Hospitals in Egypt.2015. Advances in Microbiology. 5: 269-277.

Farrell, DJ.; Morrissey, I.; De Rubeis, D.; Robbins, M. and Felmingham, D. A. (2003). UK multicentre study of the antimicrobial susceptibility of bacterial pathogens causing urinary tract infection. J Infect. 46(2): 94-100.

Gagliotti C, Balode A, Baquero F, Degener J, Grundmann H, Gür D, Jarlier V, Kahlmeter D, Monen J, Monnet D L, Rossolini G M, Suetens C, Weist K and Heuer O (2011) The EARS Net Participants (Disease Specific Contact Points for AMR), Escherichia coli and Staphylococcus aureus: bad news and good news from the European Antimicrobial Resistance Surveillance Network (EARS-Net, formerly EARSS), 2002 to 2009 (2011). Euro Surveill

Kaushlesh K Y, Shariqa M, Sarad K M, Narsuddin A M and Khurshid A (2012) Molecular characterization of cellulose degrading bacteria on the basis of 16S rRNA. International Journal of Advances in Applied Sciences 27, 80-92.

Kheiri,R. and Akhtari,L. (2016). Antimicrobial resistance and integron gene cassette arrays in commensal Escherichia coli from human and animal.

Koeleman, JG.; Stoof, J.,; Van Der Bijl, MW.; Vandenbroucke-Grauls, CM.and Savelkoul, PH. (2001). Identification of epidemic strains of acinetobacter baumannii by integrase gene PCR. J Clin Microbiol. 39:8–13.

Koo, H.J. and Woo, G.J. (2011). Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products. Int. J. Food Microbiol.,145:407-13 .

Moghaddam, M.J.M.; Mirbagheri, A.A.; Salehi,Z. and Habibzade ,S.M.(2015). Prevalence of Class 1 Integrons and Extended Spectrum

Beta Lactamases among Multi-Drug Resistant Escherichia coli

Isolates from North of Iran. Iranian Biomedical Journal. 19 (4): 233-239.

Mohammadalipour,Z.; Kargar, M. and Doosi, A. (2017). High Frequency of Class 2 and 3 Integrons Related to DrugResistance in Clinical Isolates of Diarrheagenic E.coli n Iran. Novel Biomed.5(1):30-6.

Moura , I.; Torres, C.; Somalo, S.; Igrejas,G .and Poeta,P. (2013) . Genomic Description of Antibiotic Resistance in Escherichia coli and Enterococci Isolates from Healthy Lusitano Horses. J. Equine Vet. Sci. , 33( 12) : 1057–1063.

o.Clermont J K, Christenson E, Denamur and Gordon D M (2013) The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environmental Microbiology Reports 5(1), 58–65.

Olsson-Liljequist, B. (1997). Antimicrobial Susceptibility Testing in Sweden. III. Methodology for Susceptibility Testing. Scandinavian Journal of Infectious Diseases. Supplementum. 105:13-23.

Pena,I,; Picazo, J.J; Rodriguez-Avial, C.; Rodriguez-Avial, I. (2014). Carbapenemase-producing Enterobacteriaceae in a tertiary hospital in Madrid, Spain: high percentage of colistin resistance among VIM-1 -producing Klebsiella pneumoniae ST11 isolates. Int. J. Antimicrob . Agents. 43(5):460 - 464.

Ranjbar, R.; Giammanco, GM.; Farshad, S.; Owlia, P.; Aleo, A. and Mammina, C. (2011).Serotypes, antibiotic resistance, and class 1 integrons in Salmonella isolates from pediatric cases of enteritis in Tehran,Iran. Foodborne Pathog Dis.8(4):547–53.

Rao, AN.; Barlow, M.; Clark, LA.; Boring, JR.; 3rd, Tenover, FC. and

McGowan JE. Jr. (2006).Class 1 integrons in resistant Escherichia

coli and Klebsiella spp., US hospitals. Emerg Infect Dis. 12(6): 1011-1014.

Rowe-Magnus, DA.; Guerout, AM.; Ploncard, P.; Dychinco, B.; Davies, J. and Mazel, D. (2001).The evolutionary history of chromosomal super-integrons provides an ancestry for multiresistant integrons. Proc Natl Acad Sci USA. 98:652–657.

Shams, F.; Hasani, A.; Rezaee, M.A.; Nahaie, M.R.; Hasani, A.; Haghi,M.H.S.B.; Pormohammad,A. and Arbatan,A.E.(2015). Carriage of Class 1 and 2 Integrons in Quinolone, Extended-Spectrum- β-Lactamase-Producing and Multi Drug Resistant E.coli and

K.pneumoniae: High Burden of Antibiotic Resistance. Adv Pharm Bull. 5(3): 335-342.

Shapiro, JA. (1999).Views about evolution are evolving. Am Soc Microbiol News. 65: 201-7.

Shin, H.W.; Lim, J.; Kim, S.; Kim, J.; Kwon, G.C. and Koo,S.H.(2015). Characterization of Trimethoprim-Sulfamethoxazole Resistance Genes

and Their Relatedness to Class 1 Integron and Insertion Sequence

Common Region in Gram-Negative Bacilli. J. Microbiol. Biotechnol. 25(1): 137–142.

Sung, JY. and Oh, JE. (2014).Distribution and characterization of integrons in enterobacteriaceae isolates from chickens in Korea. J Microbiol Biotechnol. 24(7): 1008-1013.

White, PA.; McIver, CJ.and Rawlinson, WD. (2001). Integrons and gene cassettes in the Enterobacteriaceae. Antimicrob Agents Chemother. 45(9): 2658-2661.

Xu, H.; Broersma, K.; Miao, V. and Davies, J. (2011). Class 1 and class 2 integrons in multidrug-resistant gram-negative bacteria isolated from the Salmon River, British Columbia. Can J Microbiol. 57:460–470.

Yang, H. (2004). Characterization of Multiple-Antimicrobial-Resistant Escherichia coli Isolates from Diseased Chickens and Swine in China. Journal of Clinical Microbiology. 42:3483-3489.

Yu, G. (2013). Role of integrons in antimicrobial resistance: A review,

African Journal of Microbiology Research. 7(15):1301-1310.

Zheng,F.; Chen,Q.; Gong,x. and Liu,Y.(2015). Comparison of the Antibiotic Resistances of the Bacteria Harboring and not Carrying Integrons.Journal of Animal and Veterinary Advances. 14(3):69-74.

Downloads

Published

2024-03-07

How to Cite

AL-Nuaeyme, H. A., & Ali, M. R. (2024). Typing and Integron Distribution among Multi-Drug Resistance Escherichia coli. Journal for Research in Applied Sciences and Biotechnology, 3(1), 232–237. https://doi.org/10.55544/jrasb.3.1.35