Study of Microbial Contamination of River Yamuna in District Baghpat and Auraiya (UP)

Authors

  • Vimlesh Kumar Department of Zoology, Janta Vedic College, Baraut, Baghpat - 250611(U.P.), INDIA
  • Devendra Pal Singh Department of Zoology, Janta Vedic College, Baraut, Baghpat - 250611(U.P.), INDIA

DOI:

https://doi.org/10.55544/jrasb.3.1.57

Keywords:

Microbial Contamination, Water Quality, Fecal Pollution, E. coli

Abstract

Microbial contamination of River Yamunain Baghpat and Auraiya district, Uttar Pradesh, India, presents a significant public health challenge due to fecal pollution from untreated sewage and agricultural runoff. This study evaluated water quality across five stations—Kurdi, KakorKalan, Katha, ShergarhGhat, and Veejalpur (Near Ayana)—using Colilert® technology and the Most Probable Number (MPN) method to detect and quantify coliforms and Escherichia coli (E. coli) as fecal contamination indicators. Sampling stations were selected to represent a gradient of anthropogenic impacts in these rural and peri-urban areas. Water samples (100 mL) were collected in sterile conditions, maintained at 4°C, and analyzed within 6 hours per standardized guidelines. Colilert® results confirmed coliform presence at all stations, with E. coli detected at Kurdi, KakorKalan, ShergarhGhat, and Veejalpur, but absent at Katha, indicating site-specific fecal inputs. MPN analysis revealed total coliforms ranging from 1200 (Katha) to 2000 (ShergarhGhat) MPN/100 mL, and E. coli from 0 (Katha) to 1100 (ShergarhGhat) MPN/100 mL.ShergarhGhat exceeded the severe risk threshold (>1000 MPN/100 mL E. coli), classifying it as very high risk, while Kurdi and KakorKalan were high risk (900–1000 MPN/100 mL E. coli), Veejalpur moderate-to-high, and Katha moderate. These findings align with prior observations linking elevated microbial loads to sewage and runoff, with Katha’s E. coli absence suggesting natural attenuation. Recommendations include routine Colilert® and MPN monitoring, pollution source control through engineered solutions, enhanced sanitation infrastructure with decentralized treatment systems, and investigation of Katha’s attenuation factors for potential replication. Immediate interventions targeting high-risk stations, alongside sustainable practices such as eco-friendly agriculture and proper waste disposal, and regulatory enforcement, are essential to mitigate health risks and improve water quality in these districts, safeguarding public health and aquatic ecosystems.

Downloads

Download data is not yet available.

References

American Public Health Association (APHA). (2017). Standard methods for the examination of water and wastewater (23rd ed.). Washington, DC: APHA.

Ashbolt, N. J., Grabow, W. O. K., &Snozzi, M. (2001). Indicators of microbial water quality. In Water quality: Guidelines, standards and health (pp. 289-316). WHO.

Bain, R., Cronk, R., Wright, J., Yang, H., Slaymaker, T., & Bartram, J. (2014). Fecal contamination of drinking-water in low- and middle-income countries. Tropical Medicine & International Health, 19(8), 974-988.

Blodgett, R. J. (2010). Bacteriological analytical manual: Most probable number determination. FDA BAM, Appendix 2.

Boehm, A. B., Shellenbarger, G. G., & Shanks, O. C. (2002). Enterococcus and E. coli as indicators of fecal pollution. Environmental Science & Technology, 36(19), 3995-4001.

Byappanahalli, M. N., Nevers, M. B., Korajkic, A., Staley, Z. R., & Harwood, V. J. (2012). Enterococci in the environment. Microbiology and Molecular Biology Reviews, 76(4), 685-706.

Cabral, J. P. S. (2010). Water microbiology: Bacterial pathogens and water. International Journal of Environmental Research and Public Health, 7(10), 3657-3703.

Chao, K. K., Chao, C. C., & Chao, W. L. (2004). Evaluation of Colilert-18 for the detection of coliforms and Escherichia coli in subtropical freshwater. Applied and Environmental Microbiology, 70(2), 1242-1244.

Converse, R. R., Blackwood, A. D., Kirs, M., Griffith, J. F., & Noble, R. T. (2009). Rapid QPCR-based assay for fecal pollution. Water Research, 43(19), 4828-4837.

Eckner, K. F. (1998). Comparison of membrane filtration and Colilert for E. coli detection. Applied and Environmental Microbiology, 64(8), 3076-3079.

Edberg, S. C., Allen, M. J., Smith, D. B., &Kriz, N. J. (1990). Enumeration of total coliforms and Escherichia coli using Colilert. Journal of the American Water Works Association, 82(8), 68-73.

Fricker, C. R., Warden, P. S., & Eldred, B. J. (1997). Evaluation of Colilert for water quality monitoring. Letters in Applied Microbiology, 25(1), 47-51.

Fujioka, R. S. (2001). Monitoring coastal marine waters for fecal indicators. Marine Pollution Bulletin, 42(2), 117-123.

Garthright, W. E., & Blodgett, R. J. (2003). FDA’s preferred MPN methods. Journal of AOAC International, 86(6), 1143-1150.

Hagedorn, C., Crozier, J. B., Mentz, K. A., Booth, A. M., & Graves, A. K. (2011). Microbial source tracking in rural watersheds. Journal of Environmental Quality, 40(3), 811-821.

Harwood, V. J., Levine, A. D., Scott, T. M., Chivukula, V., & Lukasik, J. (2005). Validity of fecal coliforms as indicators in recreational waters. Water Research, 39(14), 3167-3175.

IDEXX Laboratories. (2018). Colilert® technical manual. Westbrook, ME: IDEXX Laboratories.

Ishii, S., &Sadowsky, M. J. (2008). Escherichia coli in the environment: Implications for water quality. Microbes and Environments, 23(2), 101-108.

Jamieson, R. C., Gordon, R. J., Sharples, K. E., &Madani, A. (2004). Movement and persistence of fecal bacteria in streams. Journal of Environmental Engineering, 130(11), 1307-1314.

Kinzelman, J., Ng, C., Jackson, E., Gradus, S., & Bagley, R. (2003). Enterococci as indicators of Lake Michigan water quality. Water Research, 37(11), 2597-2606.

LeChevallier, M. W., Welch, N. J., & Smith, D. B. (1996). Full-scale studies of factors related to coliform regrowth. Applied and Environmental Microbiology, 62(7), 2201-2211.

Leclerc, H., Mossel, D. A. A., Edberg, S. C., &Struijk, C. B. (2001). Advances in the bacteriology of the coliform group. Microbiological Reviews, 65(2), 196-215.

McLellan, S. L., &Salmore, A. K. (2003). Evidence for localized bacterial loading in urban streams. Environmental Monitoring and Assessment, 81(1-3), 261-271.

Payment, P., Waite, M., &Dufour, A. (2000). Assessing microbial safety of drinking water. Water Science and Technology, 41(7), 19-24.

Rose, J. B., Epstein, P. R., Lipp, E. K., Sherman, B. H., & Bernard, S. M. (2001). Climate variability and waterborne disease. Environmental Health Perspectives, 109(Suppl 2), 211-221.

Sinton, L. W., Hall, C. H., Lynch, P. A., & Davies-Colley, R. J. (1998). Sunlight inactivation of fecal indicator bacteria. Applied and Environmental Microbiology, 64(9), 3605-3613.

Solo-Gabriele, H. M., Wolfert, M. A., Desmarais, T. R., & Palmer, C. J. (2000). Sources of Escherichia coli in a coastal subtropical environment. Applied and Environmental Microbiology, 66(1), 230-237.

Tallon, P., Magajna, B., Lofranco, C., & Leung, K. T. (2005). Microbial indicators of faecal contamination in water: A current perspective. Water, Air, & Soil Pollution, 166(1-4), 139-166.

World Health Organization (WHO). (2017). Guidelines for drinking-water quality (4th ed.). Geneva, Switzerland: WHO.

Wright, J., Gundry, S., & Conroy, R. (2006). Household drinking water in developing countries: A systematic review. Tropical Medicine & International Health, 11(5), 604-616.

Downloads

Published

2024-02-27

How to Cite

Kumar, V., & Singh, D. P. (2024). Study of Microbial Contamination of River Yamuna in District Baghpat and Auraiya (UP). Journal for Research in Applied Sciences and Biotechnology, 3(1), 383–388. https://doi.org/10.55544/jrasb.3.1.57

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.