Dual Application of Nanotechnology of Pine Needles Catalyst and Graphene-Based System for Cardioprotective Application

Omprakash Vishwanath Swami; Neha Deshwal; Vipul Samrat; Kalpana Sonwani; Vivek Kumar; Zubair Arshad; Saurabh Kumar Chauhan

doi:10.55544/jrasb.5.1.14

Authors

Omprakash Vishwanath Swami Department of Pharmaceutical Quality Assurance, Progressive Education Society's Modern College of Pharmacy Nigadi, Pune, INDIA.
Neha Deshwal Assistant professor, Department of Pharmaceutics, JB institute of pharmacy, Chota Rampur, Sahaspur, Cakrata Road, Dehradun, INDIA.
Vipul Samrat Department of Pharmacy, Sai Baba Institute of Pharmaceutical Sciences, Muzaffarpur, 843122, Bihar, INDIA. https://orcid.org/0009-0008-4083-2107
Kalpana Sonwani Head of Department, G.S.R.M Memorial College of Pharmacy, Lucknow, INDIA.
Vivek Kumar Department of Pharmacy, Sai Baba Institute of Pharmaceutical Sciences Muzaffarpur, 843122, Bihar, INDIA.
Mohd. Zubair Arshad Department of Pharmacology, Advance Institute of Biotech and Paramedical Sciences (188), Kanpur, INDIA.
Saurabh Kumar Chauhan Department of Pharmacology, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi-110062, INDIA.

DOI:

https://doi.org/10.55544/jrasb.5.1.14

Keywords:

Nanotechnology, nanocatalyst, cardioprotective, cardiovascular disease, pine needle, graphene

Abstract

The most widespread cause of death in the world, cardiovascular diseases (CVDs), require the development of new therapeutic approaches that would overcome the current constraints of management, in terms of responding to the oxidative tissue damage, as well as facilitating regeneration. The article represents a new integrative nanotechnological method that combines a pine needle-derived nanocatalyst with a graphene-based system in the context of prerequisites of cardioprotection. The platform takes advantage of a green synthesis process, in which the phytochemical-rich Pinus extract produces multi-enzyme (SOD, catalase, peroxidase) mimetic metal nanozymes. The bioactive nanocatalysts are conjugated on the functionalized graphene oxide (GO) and reduced graphene oxide (rGO) sheets, which are famous because of their high electrical conductivity, mechanical strength, and surface area. The dual-system nanohybrid that results is a synergistic combination of the ability of the graphene component to deliver therapeutics precisely, condition electrically, and provide mechanical strength with the high-potency, catalytic ability of the pine nanozyme to scavenge reactive oxygen species (ROS). The synthesis, characterization, and multi-target of this hybrid system (such as the regulation of the Nrf2 antioxidant system, NF-kB/NLRP3-mediated inflammation, and cardiomyocyte-apoptosis) are provided. In addition, we discuss the most recent applications of graphene-based materials to drug delivery, cardiac tissue engineering, and bioelectronic interfaces, which are the basis of the rationale behind this integration. This dual-uses approach is based on the groundbreaking, theranostic platform of ischemia-reperfusion injury mitigation, cardiotoxicity in relation to drugs, and myocardial repair, which is supported by emerging human safety data on graphene oxide, one of the major steps toward catalytic and regenerative cardiovascular nanomedicine.

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References

Jampilek J, Kralova K. Advances in biologically applicable graphene-based 2D nanomaterials. International journal of molecular sciences. 2022 Jun 2;23(11):6253.

Moradi S, Nargesi Azam F, Abdollahi H, Rajabifar N, Rostami A, Guzman P, Zarrintaj P, Davachi SM. Graphene-based polymeric microneedles for biomedical applications: a comprehensive review. ACS Applied Bio Materials. 2025 Feb 10;8(3):1835-61.

Samanta BC, Sutradhar N. Recent Advancement in Therapeutic Use of Chemical Compounds and Drug Delivery. Walnut Publication; 2021 Feb 22.

Basak S, Packirisamy G. Graphene‐Based Nanomaterials for Biomedical, Catalytic, and Energy Applications. ChemistrySelect. 2021 Sep 27;6(36):9669-83.

Challa AA, Saha N, Saha P. Graphene-Based Carbonaceous Materials: A Sustainable Biomaterial for Biomedical Application. InSustainable Green Biomaterials As Drug Delivery Systems 2025 Feb 19 (pp. 165-193). Cham: Springer Nature Switzerland.

Singh A, Amiji MM. Application of nanotechnology in medical diagnosis and imaging. Current opinion in biotechnology. 2022 Apr 1;74:241-6.

Ma X, Tian Y, Yang R, Wang H, Allahou LW, Chang J, Williams G, Knowles JC, Poma A. Nanotechnology in healthcare, and its safety and environmental risks. Journal of nanobiotechnology. 2024 Nov 15;22(1):715.

Sharma PK, Dorlikar S, Rawat P, Malik V, Vats N, Sharma M, Rhyee JS, Kaushik AK. Nanotechnology and its application: a review. Nanotechnology in cancer management. 2021 Jan 1:1-33.

Dong B, Yuan Y, Ding M, Bai W, Wu S, Ji Z. Efficient dual-mode luminescence from lanthanide-doped core–shell nanoarchitecture for anti-counterfeiting applications. Nanotechnology. 2020 Jun 23;31(36):365705.

Hahm D, Lim J, Kim H, Shin JW, Hwang S, Rhee S, Chang JH, Yang J, Lim CH, Jo H, Choi B. Direct patterning of colloidal quantum dots with adaptable dual-ligand surface. Nature Nanotechnology. 2022 Sep;17(9):952-8.

Wang J, Xu X, Cheng T, Gu L, Qiao R, Liang Z, Ding D, Hong H, Zheng P, Zhang Z, Zhang Z. Dual-coupling-guided epitaxial growth of wafer-scale single-crystal WS2 monolayer on vicinal a-plane sapphire. Nature Nanotechnology. 2022 Jan;17(1):33-8.

Thipe VC, Karikachery AR, Çakılkaya P, Farooq U, Genedy HH, Kaeokhamloed N, Phan DH, Rezwan R, Tezcan G, Roger E, Katti KV. Green nanotechnology—An innovative pathway towards biocompatible and medically relevant gold nanoparticles. Journal of Drug Delivery Science and Technology. 2022 Apr 1;70:103256.

Noor B, Zubair A, Shahzad R, Zahid S, Anjum S, Anjum I, Riaz MU, Soliman MM. Cerium Oxide Nanoparticles (CeO2 NPs): A Dual Approach to Therapy and Toxicity in Hepatorenal Systems. BioNanoScience. 2025 Mar;15(1):3.

Kong XP, Zhang BH, Wang J. Multiple roles of mesoporous silica in safe pesticide application by nanotechnology: A review. Journal of Agricultural and Food Chemistry. 2021 Jun 10;69(24):6735-54.

Vignesh A, Menaka C, Amal TC, Selvakumar S, Vasanth K. Exploring the dual impact of nanoparticles on human well-being: A comprehensive review of risks and benefits. Next Nanotechnology. 2025 Jan 1;8:100223.

Sui D, Wu M, Liu Y, Yang Y, Zhang H, Ma Y, Zhang L, Chen Y. High performance Li-ion capacitor fabricated with dual graphene-based materials. Nanotechnology. 2020 Oct 19;32(1):015403.

Hu D, Pan M, Yu Y, Sun A, Shi K, Qu Y, Qian Z. Application of nanotechnology for enhancing photodynamic therapy via ameliorating, neglecting, or exploiting tumor hypoxia. View. 2020 Mar;1(1):e6.

Lai CM, Xu J, Zhang BC, He SH, Shao JW. A natural product-derived nanozyme regulator induced chemo-ferroptosis dual therapy in remodeling of the tumor immune microenvironment of hepatocellular carcinoma. Chemical Engineering Journal. 2024 Feb 15;482:148976.

Franco CA, Franco CA, Zabala RD, Bahamon I, Forero A, Cortes FB. Field Applications of nanotechnology in the oil and gas industry: Recent advances and perspectives. Energy & Fuels. 2021 Nov 18;35(23):19266-87.

Afewerki S, Bassous N, Harb S, Palo-Nieto C, Ruiz-Esparza GU, Marciano FR, Webster TJ, Furtado AS, Lobo AO. Advances in dual functional antimicrobial and osteoinductive biomaterials for orthopaedic applications. Nanomedicine: Nanotechnology, Biology and Medicine. 2020 Feb 1;24:102143.

Chen C, Li Q, Wang B, Liu Q, Wang Z, Zhang B, Wang L, Zou Y, Mou Z, Ren C, You Z. Dual delivery of agmatine and microRNA-126b using agmatine-mediated DNA nanotube assemblies for acute lung injury therapy. Acta Biomaterialia. 2025 Apr 24.

Zhang R, Jin C, Xie P, Chen T. Photoresponsive and UCST-Type thermoresponsive block Copolymer-Based composite micelles for Dual-Stimuli-Triggered selective and programmable release. European Polymer Journal. 2025 Dec 24:114472.

Luo Y, Li L, Feng Y, Li R, Yang J, PeijnenburgWJ, Tu C. Quantitative tracing of uptake and transport of submicrometre plastics in crop plants using lanthanide chelates as a dual-functional tracer. Nature nanotechnology. 2022 Apr;17(4):424-31.

Wang M, Hu Z, Yang T, Pei H, Zhang F. A dual pesticide–fertilizer silicon-base nanocomposite to synergistically control fungal disease and provide nutrition. Environmental Science: Nano. 2023;10(12):3462-75.

Fu L, Ma X, Liu Y, Xu Z, Sun Z. Applying nanotechnology to boost cancer immunotherapy by promoting immunogenic cell death. Chinese Chemical Letters. 2022 Apr 1;33(4):1718-28.

Moradi S, Nargesi Azam F, Abdollahi H, Rajabifar N, Rostami A, Guzman P, Zarrintaj P, Davachi SM. Graphene-based polymeric microneedles for biomedical applications: a comprehensive review. ACS Applied Bio Materials. 2025 Feb 10;8(3):1835-61.

Challa AA, Saha N, Saha P. Graphene-Based Carbonaceous Materials: A Sustainable Biomaterial for Biomedical Application. InSustainable Green Biomaterials As Drug Delivery Systems 2025 Feb 19 (pp. 165-193). Cham: Springer Nature Switzerland.

Pandey M, Nazar R, Elella MH, Praharaj S, Makhado E, Rout D, Gilani EH. A comprehensive review of recent developments in biomedical materials based on graphene-modified bio-nanocomposites. BioNanoScience. 2025 Mar;15(1):125.

Bagheri H, Fatehi M, Mohebbi A. Graphene synthesis, characterization and applications. InGraphene from Natural Sources 2022 Jul 18 (pp. 139-176). CRC Press.

Iqbal J, Iqbal A, Mukhtar H, Jahangir K, Mashkoor Y, Zeeshan MH, Nadeem A, Ashraf A, Maqbool S, Sadiq SM, Lee KY. Cardioprotective effects of nanoparticles in cardiovascular diseases: a state-of-the-art review. Current problems in cardiology. 2023 Aug 1;48(8):101713.

Oyelaja O, Najneen T, Alamy H, Horn WL, Medina JA, Duarte LE, Yaqobi A, Farooqi P, Mohammadi R, Almadhoun MK, Khail BM. Applications of Nanotechnology in the Field of Cardiology. Cureus. 2024 Apr 11;16(4).

Elendu C, Amaechi DC, Elendu TC, Amaechi EC, Elendu ID, Omeludike JC, Omeludike EK, Onubogu NC, Ogelle EC, Meduoye OO, Oloyede PO. Essential information about nanotechnology in cardiology. Annals of Medicine and Surgery. 2025 Feb 1;87(2):748-79.

Iervolino A, Spadafora L, Spadaccio C, Iervolino V, Biondi Zoccai G, Andreotti F. Myocardial cell preservation from potential cardiotoxic drugs: the role of nanotechnologies. Pharmaceutics. 2022 Dec 27;15(1):87.

Li F, Shao H, Zhou G, Wang B, Xu Y, Liang W, Chen L. The recent applications of nanotechnology in the diagnosis and treatment of common cardiovascular diseases. Vascular Pharmacology. 2023 Oct 1;152:107200.

Oluwaseyi O, Tazkia N, Haroon A, Horn WL, Duarte LE, Adila Y, Palwasha F, Rohullah M, Khaleel IK, Bushra MK, Abed S. Applications of Nanotechnology in the Field of Cardiology. Cureus. 2024;16(4).

Sarma H, Kashyap P, Zothantluanga JH, Devi R. Nanotherapeutics of phytoantioxidants for cardiovascular diseases. Phytoantioxidants and Nanotherapeutics. 2022 Sep 12:405-31.

Cui W, Wang A, Zhao C, Zhu W. Nanotechnology in cardiovascular regenerative medicine. Frontiers in Bioengineering and Biotechnology. 2020 Oct 23;8:608844.

Jaiswal S, Anjum MM, Thakur S, Pandey P, Arya DK, Kumar A, Kaushik AS, Rajinikanth PS. Evaluation of cardioprotective effect of naringin loaded lignin nanoparticles against isoproterenol induced myocardial infarction. Journal of Drug Delivery Science and Technology. 2023 Nov 1;89:105076.

Xu N, Zhang T, Wang X, Wang L. Cardioprotective effects of plant-based silver nanoparticles: Describing a modern drug. Inorganic Chemistry Communications. 2023 Dec 1;158:111525.

Sultan K, Akku K, Abdalimov B, Mirzamuratova G, Mendybayeva A. Innovative Nanotechnologies in the Diagnosis and Treatment of Myocardial Infarction: New Horizons and Perspectives. Scientific Collection «InterConf+».(40):406-17.

Kadu M, Salve R, Gajbhiye KR, Choudhary RK, Gajbhiye V. Nanoparticle-Mediated Plant Bioactives for Cardiovascular Disease. InAdvances in Phytonanotechnology for Treatment of Various Diseases 2023 Sep 29 (pp. 199-213). CRC Press.

Houacine C, Khan I, Yousaf SS. Potential cardio-protective agents: a resveratrol review (2000-2019). Current Pharmaceutical Design. 2021 Jul 1;27(26):2943-55.

Mendez-Fernandez A, Cabrera-Fuentes HA, Velmurugan B, Irei J, Boisvert WA, Lu S, Hausenloy DJ. Nanoparticle delivery of cardioprotective therapies. Conditioning medicine. 2020 Feb;3(1):18.

Shi W, Xu Y, Wei J, Zhang X, Zhu S, Guo H, Huang Q, Qi C, Hua T, Liu Y, Yang M. Plant-derived secondary metabolites and nanotechnology: innovative strategies and emerging challenges in myocardial ischemia-reperfusion injury therapy. Frontiers in Pharmacology. 2025 May 29;16:1529478.

Soliman AG, Mahmoud B, Eldin ZE, El-Shahawy AA, Abdel-Gabbar M. Optimized synthesis characterization and protective activity of quercetin and quercetin–chitosan nanoformula against cardiotoxicity that was induced in male Wister rats via anticancer agent: doxorubicin. Cancer Nanotechnology. 2023 Dec;14(1):10.

Shakarami A, Pooria A. Gold nanoparticles in cardiovascular medicine: current applications and future directions in nano-cardiology. Annals of Medicine and Surgery. 2025 Nov 1;87(11):7356-61.

Sajjad MW, Muzamil F, Sabir M, Ashfaq UA. Regenerative Medicine and Nanotechnology Approaches against Cardiovascular Diseases: Recent Advances and Future Prospective. Current Stem Cell Research & Therapy. 2025 Jan;20(1):50-71.

Saeed S, Din SR, Khan SU, Gul R, Kiani FA, Wahab A, Zhong M. Nanoparticle: a promising player in nanomedicine and its theranostic applications for the treatment of cardiovascular diseases. Current Problems in Cardiology. 2023 May 1;48(5):101599.