Isolation and Diagnosis of Escherichia coli bacteria from Patients with Urinary Tract Infections and Bacteriosis and Studying their Sensitivity to Some Antibiotics and Nanobodies

Rania Maged Hamad; Yassien Hussain Owaied Al-Juboory; Iman Tajer Abdullah

doi:10.55544/jrasb.2.4.27

Authors

Rania Maged Hamad College of Education for Pure Sciences, Tikrit University, IRAQ. https://orcid.org/0009-0009-7042-0450
Yassien Hussain Owaied Al-Juboory College of Education for Pure Sciences, Tikrit University, IRAQ.
Iman Tajer Abdullah College of Science, Kirkuk University, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.2.4.27

Keywords:

Coliform Bacteria, Antibiotic Resistance., Nanoparticles, Bacteremia, Urinary Tract Infections

Abstract

The current study included the collection of 120 clinical samples (diuresis, blood) from patients visiting Baghdad city hospitals (Yarmouk Teaching Hospital, Central Children's Teaching Hospital, and Baghdad Teaching Hospital) for the period from November 2022 to February 2023. 30 discharge samples were collected from healthy people as a control group. The samples were within different ages and genders ranging from 14 years to 52 years. 10% of the E bacteria have been isolated coli of blood and 150% of blood based on phenotypic and culture characteristics and biochemical tests and the diagnosis was confirmed using the phytic device.

The results of the sensitivity test of bacterial isolates using the tablet method of diffusion showed that most of the isolates were resistant to the antibiotics used, where the percentage of resistance to Tetracycline (96%), (84%) Nitrofurantoin, (82%) Ceftriaxone, (66%) Meropenem, (52%) Ciprofloaxcine, (70%) Gentamicin, (80%) Amikacin, (46%) Imipenem, Nalidixic acid (40%).

The antimicrobial activity of gold and titanium nanoparticles was evaluated against 15 bacterial isolates (5 isolated from diuretic samples, 5 exit samples, 5 blood samples) using the diffusion method by gratigran. The results showed that the nanoparticles had a clear inhibitory effect against the studied isolates where the average inhibition diameters ranged between 17-22 mm and the MIC value (Minimal inhibitory concentration) was between 64-126.

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References

Al-Saadi, 2021, Nanotechnology is a newly emerging science that includes nanotechnology, the process of manufacturing and developing materials into nanoparticles, as the average sizes of nanoparticles range between 1-100 nanometers, so they can be used for applications in energy, medicine, diagnostics, optics, electronics, as well as water treatment systems.

Antao. Et T Al., 2009 , Nanotechnology may be alternative therapies because of their high effectiveness against pathogenic germs and their safe use.

Barcella et. al. ,2006, Bacteria produce organic acids as a result of fermentation of lactose and sucrose sugars.

Brown and Smith and Tille, 2017 ,IMVIC Group test results The bacteria were positive for both the Indole test and the Red Metsul test while negative for the Fox-Prosquer test.

CLSI. ,2016, According to the standard specifications contained in the bacteria are considered sensitive (S), resistant (R) or medium (I) intermediate.

Ghadi et . Al.,2014 , Modification of pH to 10 by NaoH (IN).

Hadi et . al., 2014, causes many diseases such as diarrhea, diarrheameningitis, septicemia, sepsis bacteremia and is one of the most common types of bacteria that cause urinary tract infections, as it causes about 9% of urinary tract infections in the world and is more common in childhood.

Hemraj et al., 2013, catalase positive and oxidase negative indole producer and non-citrate citrate positive for red methylation test.

Holt . et al.,1994, Diagnosis of bacterial isolates based on phenotypic characteristics including colony shape, texture, smell, medium of manconki and methyl eosine.

Kapoor at .al .,2017 ,Bacteria are characterized by having the characteristic of multiple resistance to antibiotics E. coli bacteria as a result of possessing resistance enzymes such as betalactamese enzymes that give resistance to betalactam antibodies and enzymes that give resistance to aminoclecoside and anti-ketolinics, and bacteria have other mechanisms that give them resistance to antibiotics such as changing the permeability of the cell membrane.

Levinson ,2016 ,The appearance of E. coli cells in the form of short Gram-negative bacilli .

Morello. et. Al.,2006 ,Allergy screening using antibiotic tablet method.

Rastogi et. al., 2016, Application of the Scherrer equation to measure the size of nanoparticles.

Wanger et al E. ,2017, Coli bacteria are members of the enteral family Entrobacteriaceae negative dye Cram, bacillus form, mobile and non-motile aerobic or anaerobic optional Facultative anaerobic fermentation of lactose sugar, most of which fermented Ramenose sugar and Sorbitol sugar, producing the enzyme –glucuronidase The optimum temperature for their growth is 37 °C.

Zowawi et al.,2015.' Terlizz et al.,2017 E. coli bacteria have many paraboloid factors that give bacteria antigen characteristics by producing flageller antigen H, somatic antigen (O) and capsular antigen (k) as well as cilia (pili or fimbriae) that help them adhere to the host tissues, giving them the ability to form a biofilm.