Butea monosperma Silver Nanoparticles Anticancer Activity Against MCF 7 Human Breast Cancer Cell Line

Akshay Milind Patil; Raghavendra HL; Ganesh Bapuro Janvale

doi:10.55544/jrasb.2.4.4

Authors

Akshay Milind Patil Centre for Biotechnology Pravara Institute of Medical Sciences (DU) Loni – 413736, Tal. Rahata Dist. Ahmednagar, Maharashtra, INDIA. https://orcid.org/0009-0003-5668-099X
Raghavendra HL Centre for Biotechnology Pravara Institute of Medical Sciences (DU) Loni – 413736, Tal. Rahata Dist. Ahmednagar, Maharashtra, INDIA.
Ganesh Bapuro Janvale Department of Bioinformatics, College of Agricultural Biotechnology, MPKV Rahuri, INDIA.

DOI:

https://doi.org/10.55544/jrasb.2.4.4

Keywords:

Butea monosperma, UV-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Silver nanoparticles, Anticancer activities, MCF 7 human breast cancer cell line, MTT assay

Abstract

In this research, silver nanoparticles were synthesized from Butea monosperma for in vitro cytotoxicity efficacy against MCF-7 cells. Silver nanoparticles are deemed the most positive, considering their strong volume surface region, and are of concern for study because of the improved microbial tolerance to antibiotics and medicines. Therefore, green synthesis of nanoparticles of silver using biomolecules derived from various plant sources in the form of extracts can be applied for the screening of different diseases which trigger microorganisms and for the physical and biological characterization of plant-derived silver nanoparticles. The experiment involved the green synthesis of silver nanoparticles (AgNPs) from Butea monosperma leaf extract. Biosynthesized Butea monosperma-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The intensity of peak broad range 200-800nm in UV-vis spectra, EDS test. The SEM shows the actual size of the nanoparticles. The MTT assays were carried out for cytotoxicity of various concentrations of biosynthesized silver nanoparticles. The biosynthesized silver nanoparticles showed a significant anticancer activity against both MCF-7. Our study thus revealed an excellent application of greenly synthesized silver nanoparticles. At the Concentration 80µg/ml, Sample Code A, B, C, D samples showed good percent inhibition MCF7cell line as compared to standard drug.The study also suggested the potential therapeutic use of these nanoparticles in cancer study.

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