A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications

Deepak Kumar; Amrendra Kumar; Vinay Kumar; Arjesh Raj; Raja Ram Mohan Rai; Vishal Baliyan; Nitish Kumar

doi:10.55544/jrasb.2.2.9

Authors

Deepak Kumar Scholar, Department of Quality Assurance, Roorkee College of Pharmacy, Roorkee, INDIA.
Amrendra Kumar Scholar, Department of Pharmaceutics, Roorkee College of Pharmacy, Roorkee, INDIA.
Vinay Kumar Faculty, Department of Pharmaceutics, Rajvanshi Ramashish College of Pharmacy, Bihar, INDIA.
Arjesh Raj Ph.D. Scholar, Department of Pharmacology, Mansarovar Global University, MP, INDIA.
Raja Ram Mohan Rai Faculty, Department of Pharmaceutics, Shivam College of Higher Studies Pharmacy, Bihar INDIA.
Vishal Baliyan Faculty, Department of Pharmaceutics, Roorkee College of Pharmacy, Roorkee, INDIA.
Nitish Kumar Ph.D. Scholar, Department of Pharmaceutics, Lords University Chikani, Alwar, Rajasthan, INDIA.

DOI:

https://doi.org/10.55544/jrasb.2.2.9

Keywords:

HPLC, Pharmaceutical Application, Phases, Active Ingredients

Abstract

The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highlights the need of developing and testing such methods in addition to outlining the advantages of using RP-HPLC in industries like pharmaceutical, food, and environmental analysis. As examples of more recent advancements in RP-HPLC, new stationary and mobile phases, RP-HPLC downsizing, and hyphenated methods are also discussed. This review article provides a comprehensive tool for designing, refining, and validating RP-HPLC processes.

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