Bioethanol Production from Ammonia Pretreated Rice Straw

Shaik Muzammil Pasha; P. Navya; Shaik Musfera; Y.S. Goutham; Chand Pasha

doi:10.55544/jrasb.1.5.13

Authors

Shaik Muzammil Pasha Department of Microbiology, Nizam College, Osmania University, Hyderabad, INDIA.
P. Navya Department of Microbiology, Nizam College, Osmania University, Hyderabad, INDIA.
Shaik Musfera Department of Microbiology, Shantinikethan Women’s College, Osmania University, Hyderabad, INDIA.
Y.S. Goutham Department of Microbiology, Nizam College, Osmania University, Hyderabad, INDIA.
Dr. Chand Pasha Department of Microbiology, Nizam College, Osmania University, Hyderabad, INDIA.

DOI:

https://doi.org/10.55544/jrasb.1.5.13

Keywords:

Rice straw, ammonia pretreatment, acid hydrolysis, detoxification, fermentation efficiency

Abstract

Rice straw is produced in large quantity throughout the world. Rice straw is a leading feedstock for bioethanol production. Diluted ammonia pretreatment for one week at room temperature was found to be effective pretreatment. This pretreated rice straw was acid hydrolyzed and subsequently fermented with Saccharomyces cerevisiae CP11 strain. 1.5% ammonia pretreatment at room temperature for one week resulted 82.4% delignification and 78.49% of acid hydrolysis. Acid hydrolysate was fermented with maximum ethanol concentration 5.70 % with an ethanol yield of 0.46g/g and fermentation efficiency of 90.6%. Diluted ammonia pretreatment at higher temperature has reduced delignification, saccharification and fermentation efficiency with more phenols and furfurals.

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References

Y. Wang et al. Cascading of engineered bioenergy plants and fungi sustainable for low-cost bioethanol and high-value biomaterials under green-like biomass processing. Renew. Sustain. Energy Rev. 2021; vol. 137.

Kaparaju P, Serrano M, Thansen AB, Kongian P, Angelidai I. (2009). Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour Technol, 100, 2562-2568.

Krishna, S. H., Janardhan, R. T. & Chowdary, G. V. (2001). Simultaneous saccharification and fermentation of lignocellulosic waste to ethanol using a thermotolerant yeast. Bioresource Technology, 77, 193-196.

D. Y. Tsunatu, K. G. Atiku, T. T. Samuel, B. I. Hamidu & D. I. Dahutu. (2017). Production of bioethanol from rice straw using yeast extracts peptone dextrose. Nigerian Journal of Technology, Vol. 36, No. 1, 296 – 301.

Elsayed B. Belal. (2013). Bioethanol production from rice straw residues. Brazilian Journal of Microbiology, 44, 1, 225-234.

Ko, J. K., Bak, J. S., Jung, M. W., Lee, H. J., Choi, I., Kim, T. H., & Kim, K.H. (2009). Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes. Bioresour. Technol., 100 (19), 4374-4380.

Kim, T.H., Gupta, R., & Lee, Y.Y. (2009b). Pretreatment of biomass by aqueous ammonia for bioethanol production. Methods. Mol. Biol., 581, 79-91.

Mike Lawrence, Andrew Heath & Pete Walker. (2009). Determining moisture levels in straw bale construction. Construction and Building Materials, 23, 2763–2768.

D.M.Updegraff. (1969). Semimicro determination of cellulose in biological materials. Analytical Biochemistry, 32, 420–424.

Banchorndhevakul, S. (2002). Effect of urea and urea-gamma treatments on cellulose degradation of Thai rice straw and corn stalk. Radiat. Phys. Chem., 64(5-6), 417-422.

Sulbaran-De-Ferrer, B., Aristiguieta, M., Dale, B. E., Ferrer, A., & Ojeda-De-Rodriguez, G. (2003). Enzymatic hydrolysis of ammonia-treated rice straw. Appl. Biochem. Biotechnol., 105-108, 155-164.

TAPPI Committee: TAPPI test methods. (1992). Atlanta, GA (USA): TAPPI Press.

Miller GL. (1959). Use of dinitro salicylic acid reagent for determination of reducing sugar. Anal Chem, 31, 426-428.

Singleton, V.L., Rossi, J.A. (1965). Colorimetric of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult., 16, 144-158.

Congcong Chi , Zeng Zhang , Hou-min Chang & Hasan Jameel. (2009). Determination of Furfural and Hydroxymethylfurfural Formed From Biomass Under Acidic Conditions. Journal of Wood Chemistry and Technology, 29, 4, 265-276..

Malarmathi Kavindapadi Balasubramaniam & Ravikumar Rajarathinam. (2013). Implementation of White rot fungal Pretreated Rice straw for Sustainable Bioethanol Production by Saccharomyces cerevisiae. International Journal of Engineering Research & Technology, Vol. 2, Issue 11.

Shengying Jin & Hongzhang Chen. (2007). Near-infrared analysis of the chemical composition of rice straw. Industrial Crops and Products, 26, 207–211.

M. Hashem, E. H. Ali, & R. Abdel-Basset. (2013). Recycling Rice Straw into Biofuel "Ethanol" by Saccharomyces cerevisiae and Pichia guilliermondii. J. Agr. Sci. Tech., Vol. 15, 709-721.

Hadayatkhah, A., Motamedi, H., NajafzadehVarzi, H., Ghezelbash, G., Amopour Bahnamiry, M., & Karimi, K. (2013). Improvement of hydrolysis and fermentation of sugarcane bagasse by soaking in aqueous ammonia and methanolic ammonia. Biosci. Biotechnol. Biochem., 77 (7), 1379-1383.

Kim et al. (2013). Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia. Biotechnology for Biofuels, 6, 109.

Kolusheva T & Marinova A. (2007). A study of the optimal conditions for starch hydrolysis through thermostable a-amylase. Journal of the University of Chemical Technology and Metallurgy, 42, 93-96.

Vlasenko EY, Ding H, Labavitch JM, Shoemaker SP. (1997). Enzymatic hydrolysis of pretreated rice straw. Califor Inst Food Agric Res, 59, 109-119.

Kim, T., Nghiem, N., & Hicks, K. B. (2009a). Pretreatment and fractionation of corn stover by soaking in ethanol and aqueous ammonia. Appl. Biochem. Biotechnol., 153(1-3), 171-179.

Kim, T. H., Kim, J. S., Sunwoo, C., & Lee, Y. Y. (2003). Pretreatment of corn stover by aqueous ammonia. Bioresour. Technol., 90(1), 39-47.

Kim, T. H., & Lee, Y. Y. (2005). Pretreatment and fractionation of corn stover by ammonia recycle percolation process. Bioresour. Techol., 96(18), 2007-2013.

Poornejad, N., Karimi, K., & Behzad, T. (2013). Improvement of saccharification and ethanol production from rice straw by NMMO and (BMIM) (OAc) pretreatments. Ind. Crop. Prod., 41(6), 408-413.

Li, X., Kim, T. H., & Nghiem, N. P. (2010). Bioethanol production from corn stover using aqueous ammonia pretreatment and two-phase simultaneous saccharification and fermentation (TPSSF). Bioresour. Technol., 101(15), 5910-5916.

Kim, T., & Lee, Y. (2007). Pretreatment of corn stover by soaking in aqueous ammonia at moderate temperatures. Appl. Biochem. Biotechnol., 137-140(1-12), 81-92.

S. Bhattacharyya, A K Chandel, M. Taherzadeh, C. Bhattacharjee. (2022). Enhance the bioethanol production from the extracted fermentable sugar by sonication. Journal of Phase Change Materials, 2(1).